PDF] Impact of culture vessels on micro-morphological features of in vitro Dendrobium Sabin Blue orchid

Overall, the PLBs cultured in a large 470 ml plastic vessel showed successful growth with a high growth index, high carbohydrate content, low-stress condition, and high chlorophyll content, suggesting that plastic as a culture vessel provides a good impact for culturing PLBs and has low cost. Different designs of the plant tissue culture vessel, such as size, material, and shape, may alter its microenvironment atmosphere. The present study was conducted on protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid to determine the development of PLBs on plastic and glass culture vessels of different sizes. PLBs were cultured in half-strength Murashige and Skoog (MS) medium with the same initial weight of 0.5 g in 10 replicates. The growth index of the PLBs was calculated after 11 weeks to study their growth in every vessel; additionally, biochemical analysis was performed to determine carbohydrate content, proline concentration, and photosynthesis pigments in the PLBs. Scanning electron microscopy (SEM) was performed to study stomata development on PLBs in each vessel, and histological analyses were conducted to study the cell structure. Overall, the PLBs cultured in a large 470 ml plastic vessel showed successful growth with a high growth index, high carbohydrate content, low-stress condition, and high chlorophyll content. SEM confirmed that the presence of trichome and rhizoid in PLBs cultured in the 470 ml plastic vessel. Histological analysis showed the formation of the shoot on the PLBs and the presence of starch granules. Thus, the use of plastic as a culture vessel provides a good impact for culturing PLBs and has low cost.

Precocious in vitro flowering in threatened ornamental orchid

Plants, Free Full-Text

PDF) Effects of plant growth regulators and activated charcoal on

Morphophysiology and cryopreservation of seeds of Dendrobium nobile Lindl. (Orchidaceae) at different stages of development

PDF] Physical Properties of Culture Vessels for Plant Tissue

PDF) A Novel Approach for Preliminary PVS2 Vitrification

PDF) Very low and high temperature act as stress factor on

Horticulturae, Free Full-Text

PDF) Impact of culture vessels on micro-morphological features of

PDF] Micromorphological analysis of Aranda Broga Blue orchid PLBs

PDF] Physical Properties of Culture Vessels for Plant Tissue

PDF) Dendrobium micropropagation: a review

PDF) Asymbiotic seed germination and in vitro propagation of

PDF] Impact of culture vessels on micro-morphological features of

Orchid Biochemistry, PDF, Gardening