When haemoglobin unloads oxygen, its affinity for oxygen decreases. So it should get progressively easier to release each oxygen molecule. However, in reality, it gets progressively harder to unload the oxygen although
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How does haemoglobin bind to oxygen molecules when diatomic oxygen
What is the chemical composition of haemoglobin? - Quora
Figure 32.20
Why does affinity of hemoglobin for oxygen decrease when partial pressure of oxygen decreases? - Quora
Solved Hemoglobin Binding: Hemoglobin binding and exercise
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Why does it get progressively harder for each oxygen molecule to
Solved Oxygen (O2) is normally carried bound to hemoglobin