Evaluating coverage bias in next-generation sequencing of
Whole-genome sequencing is essential to many facets of infectious disease research. However, technical limitations such as bias in coverage and tagmentation, and difficulties characterising genomic regions with extreme GC content have created significant obstacles in its use. Illumina has claimed that the recently released DNA Prep library preparation kit, formerly known as Nextera Flex, overcomes some of these limitations. This study aimed to assess bias in coverage, tagmentation, GC content, average fragment size distribution, and de novo assembly quality using both the Nextera XT and DNA Prep kits from Illumina. When performing whole-genome sequencing on Escherichia coli and where coverage bias is the main concern, the DNA Prep kit may provide higher quality results; though de novo assembly quality, tagmentation bias and GC content related bias are unlikely to improve. Based on these results, laboratories with existing workflows based on Nextera XT would see minor benefits in transitioning to the DNA Prep kit if they were primarily studying organisms with neutral GC content.
PDF] Summarizing and correcting the GC content bias in high
Sequencing biases of low-GC regions and assembly quality of entire
Comparison of different nucleosome dissociation methods in
Pathogens, Free Full-Text
Barplot showing the coverage of samples sequenced by HiScanSQ and
Marine Drugs, Free Full-Text
Evaluating coverage bias in next-generation sequencing of
Evaluating coverage bias in next-generation sequencing of
Pathogens, Free Full-Text
Frontiers The efficiency of Nextera XT tagmentation depends on G
PDF] Illuminating Choices for Library Prep: A Comparison of
PDF] Illuminating Choices for Library Prep: A Comparison of
PDF] Summarizing and correcting the GC content bias in high
of serotype predictions that lack an O antigen call by the